Unexpected observation

The dissociation rate constant of bimolecular complexes between macromolecules can be quantified by way of competition experiments and also is usually likely to follow along with first-order kinetics.When measuring k constants by contest for three complexes of both high-affinity recombinant antibody fragments with the cognate antigen and also for a single calmodulin/peptide complex, a sudden reliance between apparent dissociation speed and concentration of competition has been detected. Our outcome could be feature for macromolecules comprising two domain names and could signify a transient introduction of both domains that are included in the binding reaction, and are connected by means of a polypeptide linker.

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